ILLUMINA CLUSTER STATION AMPLIFICATION LINEARIZATION BLOCKING HYBRIDIZATION
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ILLUMINA CLUSTER STATION AMPLIFICATION LINEARIZATION BLOCKING PRIMER HYBRIDIZATION
14 DAYS DOA GUARANTEE FOR FULL REFUND .
The Cluster Station is a fluidics device that hybridizes samples onto a flow cell and amplifies them for later sequencing on the Genome Analyzer. During cluster generation, a single DNA fragment (the template) is attached to the surface of an oligonucleotide-coated flow cell and amplified to form a surface-bound colony (the cluster). Cluster generation consists of two main steps: cluster amplification followed by linearization, blocking, and primer hybridization. }
Cluster Amplification—The sample is hybridized onto the flow cell and then isothermally amplified. }
Linearization, Blocking, and Primer Hybridization—The amplified sample is prepared for sequencing using the following three processes: • Linearization—Selectively cleaves off one of the two adapter molecules from the surface of the flow cell. This ensures that subsequent primer hybridization and sequencing only occurs on one of the two possible strands within each cluster. • Blocking—Blocks the 3’ OH ends of the linearized dsDNA clusters, which prevents the addition of fluorophore substrate to anywhere except the hybridized sequencing primer. • Denaturation and Hybridization—Denatures dsDNA, leaving singlestranded templates anchored to the glass flow cell surface, and then hybridizes sequencing primers onto the linearized and blocked clusters, one primer per cluster. After this step, the flow cell is ready for sequencing. With the exception of preparing reagents and template DNA, all cluster generation steps are performed on the Cluster Station
***Please check out the manual 1 for more information.***
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